Projet ANR DILI on CHIP

DILI-on-chip (Drug Induced Liver Injury) addresses the development of a microfluidic device to assess in vitro adverse drug reactions, on a human-like liver lobule. From a unique donor, hiPSCs (human induced pluripotent stem cells) will be differentiated to obtain the different types of cells, hepatocytes as well as non-parenchymal cells, that compose the liver lobule. Hepatocytes forming the hepatic cord, bordered by endothelial cells forming the sinusoid, and cholangiocytes forming the bile ducts will be organized in 3D on the chip, in order to get a functional unit mimicking the Hering channel.