Preprints

BIN1 genetic risk factor for Alzheimer is sufficient to induce early structural tract alterations in entorhinal cortex-dentate gyrus pathway and related hippocampal multi-scale impairments, Daudin R, Marechal D, Wang Q ¬, Abe Y , Bourg N , Sartori M , Loe-Mie Y , Lipecka J , Guerrera C , McKenzie A , Potier B , Dutar P , Viard, J , Lepagnol-Bestel A.M , Winkeler A , Hindié V , Birling MC, Lindner L, Chevalier C, Pavlovic G, Dupuis G, Lévêque-Fort, S, Diaz J, Davenas, E, Dembele D , Laporte J, Thibault-Carpentier C, Malissen B, Rain J.C, Ciobanu L, Le Bihan D, Zhang B , Herault Y http://dx.doi.org/10.1101/437228doi

Published

Fast scanned widefield scheme provides tunable and uniform illumination for optimized SMLM on large fields of view
Adrien Mau, Karoline Friedl, Christophe Leterrier, Nicolas Bourg, Sandrine Lévêque-Fort
doi: https://doi.org/10.1101/2020.05.08.083774 now published in Nature Communications 2021

Molecular motion and tridimensional nanoscale localization of kindlin control integrin activation in focal adhesions
Thomas Orré, Zeynep Karatas, Birgit Kastberger, Clément Cabriel, Ralph T. Böttcher, Sandrine Lévêque-Fort, Jean-Baptiste Sibarita, Reinhard Fässler, Bernhard Wehrle-Haller, Olivier Rossier, Grégory Giannone doi: https://doi.org/10.1101/2020.08.06.239731 now published in Nature Communications 2021

48- Nanometric axial localization of single fluorescent molecules with modulated excitation. P. Jouchet, C. Cabriel, N. Bourg, M. Bardou, C. Poüs, E. Fort, S. Lévêque-Fort, Biorxiv 2019, https://doi.org/10.1101/865865
now published in Nature Photonics,(2021). https://doi.org/10.1038/s41566-020-00749-9

47 STED Microscopy with Nanometer Axial Sectioning, Siddharth Sivankutty Iván Coto Hernández, Nicolas Bourg, Guillaume Dupuis and Sandrine Lévêque-Fort, Methods, 2020

46- Combining 3D single molecule localization strategies for reproducible bioimaging
Clément Cabriel, Nicolas Bourg, Pierre Jouchet, Guillaume Dupuis, Christophe Leterrier, Aurélie Baron, Marie-Ange Badet-Denisot, Boris Vauzeilles, Emmanuel Fort, Sandrine Lévêque-Fort, preprint sur bioRxiv 385799; doi: https://doi.org/10.1101/385799, Avril 2019 Nature Communications

45- Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus, Rym Boudjemaa, Clément Cabriel, Florence Dubois-Brissonnet, Nicolas Bourg, Guillaume Dupuis, Alexandra Gruss, Sandrine Lévêque-Fort, Romain Briandet, Marie-Pierre Fontaine-Aupart, Karine Steenkeste, Antimicrobial Agents and Chemotherapy 7/2018, Volume 62, Issue 7

44- Aberration-accounting calibration for 3D single-molecule localization microscopy Cabriel, C. Bourg, N., Dupuis, G. Lévêque-Fort,S. 2018 Optics Letters 43(2) 174-177

43- Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring, Bouissou A., Proag, A., Bourg N., Pingris K., Cabriel C., Balor, S., Mangeat T., Thibault, C., Vieu C., Dupuis G., Fort E., Lévêque-Fort S., Maridonneau-Parini I., Poincloux R., ACS Nano 2017, 11 (4), pp 4028–4040 Cover

42- Direct Optical Nanoscopy by Axially Localized Detection, N. Bourg, C. Mayet, G. Dupuis, T. Barroca, P. Bon, S.Lécart, E. Fort and S. Lévêque-Fort. Nature Photonics Août 2015.

 41- Three dimensional nanometer localization of nanoparticles to enhance super-resolution microscopy P. Bon, N. Bourg, S. Lécart, S. Monneret, E. Fort, J. Wenger, and S. Lévêque-Fort. Nature communications

 40- Huntingtin proteolysis causes toxicity through dynamin 1 dysregulation, Marie-thérèse El-Daher, Emilie Hangen, raul pardo, Ghislaine Poizat, Ismael Al-Ramahi, Nicolas Bourg, Sylvie Souquere, Céline Mayet, Gerard Pierron, Sandrine Leveque-Fort, Juan Botas, Sandrine Humbert. Frédéric Saudou,, EMBO journal

 39- De la microscopie à la nanoscopie de fluorescence Nicolas Bourg, Siddharth Sivankutty, Guillaume Dupuis, Actualité chimique, Juin-Juillet 2015

 38-Fluorescence lifetime and blinking of individual semiconductor nanocrystals on graphene Rogez,, H. Yang, Eric Le Moal, Sandrine Lévêque-Fort, Elizabeth Boer-Duchemin, Fei Yao, Young-Hee Lee, Yang Zhang, David Wegner, Niko Hildebrandt, Mathieu Kociak, Andrew Mayne, and Gérald Dujardin J. Phys. Chem. C Aout 2014

 37-Fast label-free cytoskeletal network imaging in living mammalian cells Pierre Bon, Sandrine Lécart, Emmanuel Fort, Sandrine Lévêque-Fort, Biophysical Journal, 106,8, pp 1588-1595   APR 15 2014

 36-Confocal Supercritical Angle Microscopy for cell membrane imaging, Siddharth Sivankutty, Thomas Barroca, Céline Mayet, Guillaume Dupuis, Emmanuel Fort,and Sandrine Lévêque-Fort. Optics Letters, 1^er Fev. 2014

 35- Label-free evanescent microscopy for membrane nano-tomography in living cells Pierre Bon, Thomas Barroca, Sandrine Lévêque-Fort, Emmanuel Fort Journal of Biophotonics Nov 2013

34- PEGylated Luminescent Gold Nanoclusters: Synthesis, Characterization, Bioconjugation, and Application to One- and Two-Photon Cellular Imaging Oh, E., Fatemi, F. K., Currie, M., Delehanty, J. B., Pons, T., Fragola, A., Lévêque-Fort, S., Goswami, R., Susumu, K., Huston, A. L. and Medintz, I. L. Part. Part. Syst. Charact.. doi: 10.1002/ppsc.201200140 (2013).

33- Homodimerization of Amyloid Precursor Protein at the plasma membrane : a homoFRET study by time resolved fluorescence anisotropy imaging V.Devauges, C.Marquer , S. Lécart, J.C. Cossec, M.C. Potier E. Fort, K. Suhling and S. Lévêque-Fort. PLOS One, 4 septembre 2012.

32- Full-Field Near-Field Optical Microscope for Cell Imaging Barroca, K. Balaa, S. Leveque-Fort, and E. Fort. Physical Review Letters 108, 21 Mai 22 2012

31- Simultaneous optically sectioned fluorescence and optical coherence microscopy with full-field illumination Makhlouf, K. Perronet, G. Dupuis, S. Leveque-Fort and A. Dubois Optics Letters, 37, 10   p 1613-1615   MAY 15 2012           

30- Determination of lipid raft partitioning of fluorescently-tagged probes in living cells by Fluorescence Correlation Spectroscopy (FCS). Marquer, S. Leveque-Fort, M.C. Potier, Journal of visualized experiments : JoVE 62, p3513   DOI: 10.3791/3513   Avril 2012

29- Full-field supercritical angle fluorescence microscopy for live cell imaging Barroca, K. Balaa, J. Delahaye, S. Leveque-Fort, and E. Fort Optics Letters Volume: 36   Issue: 16   p: 3051-3053   Aug 15 2011

28– Local cholesterol increase triggers APP-Bace1 clustering in lipid rafts and rapid endocytosis. C.Marquer, V. Devauges, J.C. Cossec, G. Liot, S. Lécart, F. Saudou, C. Duyckaerts, S. Lévêque-Fort¹ and Marie-Claude Potier¹ (¹ contribution égale)  FASEB 25, 4   p 1295-1305   APR 2011

27- Nanoroughened plasmonic films for enhanced biosensing detection. E. Le Moal, S. Leveque-Fort, M.C. Potier and E. Fort  Nanotechnology   20 (22) p 225502   2009

26- Metallic films and nanostructures for molecular fluorescence enhancement E. Le Moal, E. Fort, E; S. Leveque-Fort ACTUALITE CHIMIQUE   332   pp: 36-44   2009

25- Detection of human bladder cancer cell resistance to MVAC chemotherapy by fluorescence imaging Eschwege , A. Deniset-Besseau, S. Ferlicot, S.Lecart, S. Leveque-Fort, BA Lwaleed , G. Benoit, S. Droupy, J. Hubert, M.P. Fontaine-Aupart EUROPEAN UROLOGY SUPPLEMENTS   8 (4)   p 152 2009

24- Time-gated total internal reflection fluorescence microscopy with a supercontinuum excitation source P. Blandin, S. Lévêque-Fort, S. Lécart, J.C. Cossec, M.C. Potier, Z. Lenkei, F. Druon, et P. Georges Applied Optics 48 (2) 553-559 (Janvier 2009)

23- Picosecond polarized supercontinuum generation controlled by intermodal four-wave mixing for fluorescence lifetime imaging microscopy P. Blandin, F. Druon, M. Hanna, S. Lévêque-Fort, C. Lesvigne, V. Couderc, P. Leproux, A. Tonello, P. Georges Optics Express 16, p 18844-18849 (2008)

22- Three-dimensional time-resolved fluorescence imaging by multifocal multiphoton microscopy for a photosensitizer study in living cells  Deniset-Besseau, S. Lévêque-Fort, M. P. Fontaine-Aupart, G. Roger, and P. Georges Applied Optics, Vol. 46, Issue 33, pp. 8045-8051 novembre 2007

21– Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis Steenkeste, S. Lecart, A. Deniset, P. Pernot, P. Eschwege, S. Ferlicot, S. Leveque-Fort, R. Briandet, M.P. Fontaine-Aupart, Photochemistry and photobiology 83 (5): 1157-1166 SEP-OCT 2007

20- Mirror slides for high-sensitivity cell and tissue fluorescence imaging Le Moal , E. Fort, S. Leveque-Fort, A. Janin, H. Murata, F.P. Cordelieres, M.P. Fontaine-Aupart, C. Ricolleau  Journal of Biomedical optics 12 (2): Art. No. 024030 MAR-APR 2007

19- Enhanced fluorescence cell imaging with metal-coated slides  Le Moal, E. Fort, S. Leveque-Fort, F.P. Cordelieres, M.P. Fontaine-Aupart, C. Ricolleau Biophysical journal 92 (6): 2150-2161 MAR 15 2007

18- Impulsional Nd : YVO4 laser at 914 nm pumped by diode Blandin, F. Druon, F. Balembois, P. Georges, S. Leveque-Fort, M.P. Fontaine-Aupart Journal de Physique IV 135: 249-250 OCT 2006

 17-Diode-pumped passively mode-locked Nd:YVO4 laser at 914 nm P. Blandin, F. Druon, F. Balembois, P. Georges, S. Lévêque-Fort, M. P. Fontaine-Aupart Optics Letters, Vol. 31 Issue 2 Page 214 (January 2006)

16- In situ measurements of viral particles diffusion inside mucoid biofilms,Lacroix-Gueu P., R. Briandet, S. Lévêque-Fort, M.N. Bellon-Fontaine, M.P. Fontaine-Aupart , Comptes-rendus de Biologie ,328,1065-1072,(2005)

15-Fluorescence lifetime imaging with a low repetition rate passively mode-locked diode-pumped Nd:YVO4 oscillator  S. Lévêque-Fort, D.N. Papadopoulos, S. Forget, F. Balembois et P. Georges, Optics Letters Volume 30, Issue 2, 168-170 (2005)

14-Fluorescence lifetime imaging with a multifocal two photon microscope S. Lévêque-Fort, M.P. Fontaine-Aupart, G. Roger, P. Georges Optics Letters, Volume Volume 29, Issue 24 (2004)

13-Time-resolved fluorescence anisotropy imaging applied to live cells Klaus Suhling, Jan Siegel, Peter M. P. Lanigan, Sandrine Lévêque-Fort, Stephen E. D. Webb, David Phillips, Daniel M. Davis, Paul M. W. French  Optics Letters, Volume 29, Issue 6, 584-586 March 2004

12-Studying biological tissue with fluorescence lifetime imaging: microscopy, endoscopy, and complex decay profiles J. Siegel, D.S. Elson, S.E.D. Webb, K.C. Benny Lee, A. Vlandas, S. Lévêque-Fort, M. J. Lever, P.J.Tadrous, G.W.H. Stamp, A. Wallace, A. Sandison, T.F. Watson, F. Alavrez AND P. M. W. French Applied Optics, feature issue, 42, (16), 2995-3004 (2003).

11-Wide-field time-resolved fluorescence anisotropy imaging (TR-FAIM) – Imaging the mobility of a fluorophore J. Siegel, K. Sulhing, S. Lévêque-Fort, Y. Sabharwal, S.E.D. Webb, D.M. Davis, D. Phillipps, P.M.W. French Review of Scientific Instruments, 74, 182-192 (2003).
also selected for publication in: Virtual Journal of Biological Physics Research, February 1, 2003 and also in: Virtual Journal of Ultrafast Science, February 2003

10-Imaging the environment of green fluorescent protein  K. Sulhing , J. Siegel, D. Phillips, P.M.W. French, S. Lévêque-Fort, S.E.D. Webb,D.M. Davis Biophysical Journal, 83, 3589-3595 (2002).
9-Microscope lifetime system for microscopy and multi-well plate imaging using a blue picosecond diode laser  D. S. Elson, , J. Siegel, S. E. D. Webb S. Lévêque-Fort, M. J. Lever and P. M. W. French Optics Letters (Aout 2002)
8-Wavelength resolved 3D fluorescence lifetime imaging  S. E. D. Webb,S. Lévêque-Fort, D. S. Elson, J. Siegel, K. Suhling, M. J. Lever and P. M. W. French, Journal of Fluorescence Juin 2002
7-A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning S. E. D. Webb, Y. Gu, S. Lévêque-Fort, J. Siegel, M. J. Cole, K. Dowling, R. Jones and P. M. W. French;M. A. A. Neil, R. Juškaitis, L. O. D. Sucharov and T. Wilson; M. J. Lever
Review of scientific instrument. Avril 2002
6-In-situ local tissue characterization by backscattering acousto-optic imaging S. Lévêque-Fort, J. Selb, L. Pottier and A.C. Boccara, Optics Communications 2002.

5-3D acousto-optic modulated-speckle imaging in biological tissues J. Selb, S. Lévêque-Fort, L. Pottier and C. Boccara, Compte Rendu de l’Académie des Science 2001
4-Whole-field “5D” fluorescence microscopy J. Siegel, D.S. Elson, S.E.D. Webb, D. Parsons-Karavassilis, S. Lévêque-Fort, M.J. Cole, M. J. Lever and P.M.W. French; M. A. A. Neil, R. Juškaitis, L. O. Sucharov and T. Wilson Opt. Lett.,26, 1338 (2001) .
3-Application of the stretched exponential function to fluorescence lifetime imaging K.C. Benny Lee, J. Siegel, S.E.D. Webb, S. Lévêque-Fort, M.J. Cole, R. Jones, K. Dowling, P.M.W. French and J. Lever, Biophysical Journal (Septembre 2001)
2-Three dimensional acousto-optic imaging in biological tissues with parallel signal processing S. Lévêque-Fort, Applied Optics Vol. 40, No 7, 1029-1036 (2001).
1-Ultrasonic Tagging of Photons Paths in Scattering Media : Parallel Speckle Modulation Processing  S. Lévêque, A.C. Boccara, M. Lebec, and H. Saint-Jalmes,
Opt. Lett., 24, 181 (1999)
Patents (6)

 Fluorescence detection device  Lévêque-Fort S., Fontaine-Aupart M. P., Fort E., Le Moal E. et Ricolleau C, Brevet CNRS 05290480 déposé en Mars 2005.Extension internationale en Mars 2006.

Procédé de détection de nanoparticules et ses applications Fort E, Lévêque-Fort S., Le Moal E. et Ricolleau C français N°0511384, déposé le 9 Novembre 2005. Extension internationale en 2008.

 Microscopie de fluorescence champ proche plein champ K.Balaa, J .Delahaye, S. Lévêque-Fort, E. Fort Dépôt juin 2009 Patent FR 09 51816

Méthode d’observation de l’émission de lumière d’un échantillon par microscopie optique dynamique, E. Fort, S. Lévêque-Fort, T. Barroca, K. Balaa IPN : FR1058913 ; Date: 28 Octobre 2010.

Méthode et dispositif optique de télémetrie. P. Bon, E. Fort, S. Lévêque-Fort, FR1459500 Octobre 2014

Système et procédé de mesure d’un paramètre physique d’un milieu, S. Lévêque-Fort, E. Fort, N. Bourg 25 juillet 2016 FR1657130