{"id":56,"date":"2015-10-06T09:39:02","date_gmt":"2015-10-06T08:39:02","guid":{"rendered":"http:\/\/hebergement.u-psud.fr\/leveque-fort\/?page_id=56"},"modified":"2020-09-15T17:45:00","modified_gmt":"2020-09-15T16:45:00","slug":"sted","status":"publish","type":"page","link":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=56","title":{"rendered":"STED"},"content":{"rendered":"<div class=\"crayon article-texte-1539 \">\n<h3 class=\"spip\">Stimulated Emission Depletion microscopy<\/h3>\n<p>The STED microscope (Stimulated Emission Depletion) enables selectively depletion of the fluorophores on the outpart of the focal point (see fig), which greatly reduce the volume where fluorescence can be emitted, and so provide a resolution of ten nanometers. This technique developed by Stefan Hell team is slowing spreading in the community as the technical implementation tends to be tedious.<br \/>\n<a href=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/STED_principe3.jpg\"><img loading=\"lazy\" src=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/STED_principe3.jpg\" alt=\"STED_principe3\" width=\"600\" height=\"358\" class=\"aligncenter size-full wp-image-210\" srcset=\"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/STED_principe3.jpg 600w, https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/STED_principe3-300x179.jpg 300w\" sizes=\"(max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p>In collaboration with CPBM and the group of ELSA from Intsitut d\u2019optique, we have developed a system offering tunability fro excitation\/depletion beam, associated with dynamic detection (FLIM \/ FCS). Our device built up by Siddharth Sivankutty during is thesis currently can achieve a lateral resolution of about 40 nm, as illustrated in fluorescent nanospheres images and the associated profile (see fig. below). Experiments on actin network allow to highlight that the gain in resolution is preserved in cell.<img loading=\"lazy\" src=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/sted.jpg\" alt=\"sted\" width=\"485\" height=\"238\" class=\"aligncenter size-full wp-image-212\" srcset=\"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/sted.jpg 485w, https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/sted-300x147.jpg 300w\" sizes=\"(max-width: 485px) 100vw, 485px\" \/><\/a>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Stimulated Emission Depletion microscopy The STED microscope (Stimulated Emission Depletion) enables selectively depletion of the fluorophores on the outpart of the focal point (see fig), which greatly reduce the volume where fluorescence can be emitted, and so provide a resolution of ten nanometers. This technique developed by Stefan Hell team is slowing spreading in the [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":60,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.7.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=56\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"STED - From Microscopy to Nanoscopy\" \/>\n<meta property=\"og:description\" content=\"Stimulated Emission Depletion microscopy The STED microscope (Stimulated Emission Depletion) enables selectively depletion of the fluorophores on the outpart of the focal point (see fig), which greatly reduce the volume where fluorescence can be emitted, and so provide a resolution of ten nanometers. This technique developed by Stefan Hell team is slowing spreading in the [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=56\" \/>\n<meta property=\"og:site_name\" content=\"From Microscopy to Nanoscopy\" \/>\n<meta property=\"article:modified_time\" content=\"2020-09-15T16:45:00+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/STED_principe3.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/#website\",\"url\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/\",\"name\":\"From Microscopy to Nanoscopy\",\"description\":\"Sandrine L\\u00e9v\\u00eaque-Fort - NanoBio Team @ ISMO (CNRS\/ Univ. 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This technique developed by Stefan Hell team is slowing spreading in the [&hellip;]","og_url":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=56","og_site_name":"From Microscopy to Nanoscopy","article_modified_time":"2020-09-15T16:45:00+00:00","og_image":[{"url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/STED_principe3.jpg"}],"twitter_card":"summary_large_image","twitter_misc":{"Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebSite","@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/#website","url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/","name":"From Microscopy to Nanoscopy","description":"Sandrine L\u00e9v\u00eaque-Fort - NanoBio Team @ ISMO (CNRS\/ Univ. 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