![\"centrel_mask_saf\"](\"http:\/\/hebergement.u-psud.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/centrel_mask_saf.png\")
<\/a><\/p>\nWe observed a slight loss in the lateral resolution that is due to the larger PSF of the fluorophores with emission dipoles orthogonal to the surface.<\/p>\n
Nevertheless, the quality of the images permits us to easily resolve the structures of the membrane. Besides, image quality could be further improved by using deconvolution techniques. The axial sectioning is of the order of 100 nm. Moreover, penetration depth and lateral resolution performances are maintained fig.\u00a0 Images of HEK cells in (a) UAF + SAF mode and in b)\u00a0 Central mask-SAF mode.<\/p>\n This work has been developped during Thomas Barocca thesis.<\/p>\n Related publication : Full-field supercritical angle fluorescence microscopy for live cell imaging\u00a0 T. <\/strong>Barroca, K. Balaa, J. Delahaye, S. Leveque-Fort, and E. Fort Optics Letters Volume: 36\u00a0\u00a0 Issue: 16\u00a0\u00a0 p: 3051-3053\u00a0\u00a0 Aug 15 2011<\/p>\n <\/p>\n","protected":false},"excerpt":{"rendered":" Central mask filtering –\u00a0 SAF only detection A first approach to discrimante between supercritical angle fluorescence (SAF) emission and undercritical emission (UAF), is to introduce a cnetral blocking mask in a conjugated plane of the BFP of a commercial objective to remove UAF components. We observed a slight loss in the lateral resolution that is […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":27,"menu_order":1,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"yoast_head":"\n\n\n\n\n\n\n\n\n\n\n\n\n\t\n<\/a><\/p>\n
\nthroughout the whole image. The central mask-SAF technique allows one to perform the observation of the whole cell and its membrane simultaneously at low cost using a standard
\nepifluorescence microscope and an incoherent source that provides an homogeneous illumination all over the sample.<\/p>\n<\/a><\/p>\n