{"id":75,"date":"2015-10-06T10:38:01","date_gmt":"2015-10-06T09:38:01","guid":{"rendered":"http:\/\/hebergement.u-psud.fr\/leveque-fort\/?page_id=75"},"modified":"2021-01-25T00:07:58","modified_gmt":"2021-01-24T23:07:58","slug":"confocal-saf","status":"publish","type":"page","link":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75","title":{"rendered":"Confocal &#8211; SAF"},"content":{"rendered":"<p>By blocking a the detection the undercritical emission (UAF), only the SAF emission is collected to reconstruct the confocal (or the STED) image. Only the detection of the microscope is modified to introduce the filtering of the UAF, and as can be seen on the images of microtubules, deeper ones disappear in the SAF image compared to the confocal image.<\/p>\n<p><a href=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png\"><img loading=\"lazy\" src=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png\" alt=\"confocal_SAF\" width=\"1470\" height=\"877\" class=\"aligncenter size-full wp-image-239\" srcset=\"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png 1470w, https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF-300x179.png 300w, https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF-1024x611.png 1024w, https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF-624x372.png 624w\" sizes=\"(max-width: 1470px) 100vw, 1470px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>By blocking a the detection the undercritical emission (UAF), only the SAF emission is collected to reconstruct the confocal (or the STED) image. Only the detection of the microscope is modified to introduce the filtering of the UAF, and as can be seen on the images of microtubules, deeper ones disappear in the SAF image [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":27,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v17.7.1 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Confocal - SAF - From Microscopy to Nanoscopy\" \/>\n<meta property=\"og:description\" content=\"By blocking a the detection the undercritical emission (UAF), only the SAF emission is collected to reconstruct the confocal (or the STED) image. Only the detection of the microscope is modified to introduce the filtering of the UAF, and as can be seen on the images of microtubules, deeper ones disappear in the SAF image [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75\" \/>\n<meta property=\"og:site_name\" content=\"From Microscopy to Nanoscopy\" \/>\n<meta property=\"article:modified_time\" content=\"2021-01-24T23:07:58+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/#website\",\"url\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/\",\"name\":\"From Microscopy to Nanoscopy\",\"description\":\"Sandrine L\\u00e9v\\u00eaque-Fort - NanoBio Team @ ISMO (CNRS\/ Univ. Paris Saclay)\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"ImageObject\",\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#primaryimage\",\"inLanguage\":\"en-US\",\"url\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png\",\"contentUrl\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png\"},{\"@type\":\"WebPage\",\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#webpage\",\"url\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75\",\"name\":\"Confocal - SAF - From Microscopy to Nanoscopy\",\"isPartOf\":{\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/#website\"},\"primaryImageOfPage\":{\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#primaryimage\"},\"datePublished\":\"2015-10-06T09:38:01+00:00\",\"dateModified\":\"2021-01-24T23:07:58+00:00\",\"breadcrumb\":{\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"SAF\",\"item\":\"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=27\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"Confocal &#8211; SAF\"}]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75","og_locale":"en_US","og_type":"article","og_title":"Confocal - SAF - From Microscopy to Nanoscopy","og_description":"By blocking a the detection the undercritical emission (UAF), only the SAF emission is collected to reconstruct the confocal (or the STED) image. Only the detection of the microscope is modified to introduce the filtering of the UAF, and as can be seen on the images of microtubules, deeper ones disappear in the SAF image [&hellip;]","og_url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75","og_site_name":"From Microscopy to Nanoscopy","article_modified_time":"2021-01-24T23:07:58+00:00","og_image":[{"url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png"}],"twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebSite","@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/#website","url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/","name":"From Microscopy to Nanoscopy","description":"Sandrine L\u00e9v\u00eaque-Fort - NanoBio Team @ ISMO (CNRS\/ Univ. Paris Saclay)","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?s={search_term_string}"},"query-input":"required name=search_term_string"}],"inLanguage":"en-US"},{"@type":"ImageObject","@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#primaryimage","inLanguage":"en-US","url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png","contentUrl":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/wp-content\/uploads\/2015\/10\/confocal_SAF.png"},{"@type":"WebPage","@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#webpage","url":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75","name":"Confocal - SAF - From Microscopy to Nanoscopy","isPartOf":{"@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/#website"},"primaryImageOfPage":{"@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#primaryimage"},"datePublished":"2015-10-06T09:38:01+00:00","dateModified":"2021-01-24T23:07:58+00:00","breadcrumb":{"@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75"]}]},{"@type":"BreadcrumbList","@id":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=75#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/"},{"@type":"ListItem","position":2,"name":"SAF","item":"http:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/?page_id=27"},{"@type":"ListItem","position":3,"name":"Confocal &#8211; SAF"}]}]}},"_links":{"self":[{"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/pages\/75"}],"collection":[{"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=75"}],"version-history":[{"count":4,"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/pages\/75\/revisions"}],"predecessor-version":[{"id":243,"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/pages\/75\/revisions\/243"}],"up":[{"embeddable":true,"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=\/wp\/v2\/pages\/27"}],"wp:attachment":[{"href":"https:\/\/hebergement.universite-paris-saclay.fr\/leveque-fort\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=75"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}