Our research develops advanced fluorescence microscopy methods to observe biological systems with nanometer precision in living cells.
We focus on single-molecule localization microscopy (SMLM) and develop new strategies for 3D nanoscopy, including approaches based on supercritical angle fluorescence (SAF) detection and time-modulated localization methods such as ModLoc.
Within the ERC TimeNanoLive project, we explore new concepts for single-molecule localization and time-resolved nanoscopy.
In parallel, we develop fluorescence lifetime imaging microscopy (FLIM) approaches to extract functional information from biological systems.
Our long-term goal is to combine 3D structural nanoscopy with functional imaging, enabling the study of molecular organization and dynamics in living cells at the nanoscale.